Off-axis digital holographic microscopy: practical design parameters for operating at diffraction limit
نویسندگان
چکیده
منابع مشابه
Off-axis digital holographic microscopy: practical design parameters for operating at diffraction limit.
The utilization of microscope objectives (MOs) in digital holographic microscopy (DHM) has associated effects that are not present in conventional optical microscopy. The remaining phase curvature, which can ruin the quantitative phase imaging, is the most evident and analyzed. As phase imaging is considered, this interest has made possible the development of different methods of overcoming its...
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Automated label-free quantitative imaging of biological samples can greatly benefit high throughput diseases diagnosis. Digital holographic microscopy (DHM) is a powerful quantitative label-free imaging tool that retrieves structural details of cellular samples non-invasively. In off-axis DHM, a proper spatial filtering window in Fourier space is crucial to the quality of reconstructed phase im...
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This Letter reports a demonstration of off-axis compressed holography in low-light level imaging conditions. An acquisition protocol relying on a single exposure of a randomly undersampled diffraction map of the optical field, recorded in the high heterodyne gain regime, is proposed. The image acquisition scheme is based on compressed sensing, a theory establishing that near-exact recovery of a...
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Recent advances in digital technologies, such as high-speed computers and large-format digital imagers, have led to a burgeoning interest in the science and engineering of digital holographic microscopy (DHM). Here we report on a novel off-axis DHM, based on a twin-beam optical design, which avoids the limitations of prior systems, and provides many advantages, including compactness, intrinsic ...
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Holographic rendering of off-axis intensity digital holograms is discussed. A review of some of the main numerical processing methods, based either on the Fourier transform interpretation of the propagation integral or on its linear system counterpart, is reported. Less common methods such as adjustable magnification reconstruction schemes and Fresnelet decomposition are presented and applied t...
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ژورنال
عنوان ژورنال: Applied Optics
سال: 2014
ISSN: 1559-128X,2155-3165
DOI: 10.1364/ao.53.002058